Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 297 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.892, 126.676, 76.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| Rwork | 0.175 |
| R-free | 0.25100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.600 * |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.128 |
| Total number of observations | 65934 * |
| Number of reflections | 33952 |
| Completeness [%] | 82.0 |
| Redundancy | 1.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 0.05 (M) | |
| 3 | 1 | drop | sodium chloride | 0.2 (M) | |
| 4 | 1 | drop | sodium azide | 0.02 (%) | |
| 5 | 1 | drop | Gal-beta1-3GalNAc | 1.5 (mM) | |
| 6 | 1 | drop | PEG8000 | 12 (%(w/v)) | |
| 7 | 1 | reservoir | PEG8000 | 40 (%(w/v)) | |
| 8 | 1 | reservoir | sodium phosphate | 0.05 (M) |
