Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-01 |
| Detector | RIGAKU |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.500, 64.500, 105.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.32100 |
| Structure solution method | DIFFERENCE FOURIER |
| Starting model (for MR) | APO-DTXR (PDB ENTRY 1DPR) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.400 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.000 |
| High resolution limit [Å] | 2.320 |
| Rmerge | 0.041 |
| Number of reflections | 9464 |
| Completeness [%] | 80.6 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA mixture | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 100 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 6-12 (%) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | MES | 100 (mM) |
