2TCT
THE COMPLEX FORMED BETWEEN TET REPRESSOR AND TETRACYCLINE-MG2+ REVEALS MECHANISM OF ANTIBIOTIC RESISTANCE
Replaces: 1TCTExperimental procedure
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.780, 68.780, 181.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.173 * |
| Rwork | 0.180 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 20.100 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 14105 |
| Completeness [%] | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 * | 18 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.2 (mM) | |
| 2 | 1 | drop | Tc | 1 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 35 (%sat) | |
| 5 | 1 | drop | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 7 | 1 | reservoir | ammonium sulfate | 5 (mM) | |
| 8 | 1 | reservoir | Tris-HCl | 50 (mM) |
