2SNW

SINDBIS VIRUS CAPSID PROTEIN, TYPE3 CRYSTAL FORM

Replaces:  1SNW

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	285
Detector technology	AREA DETECTOR
Collection date	1992-02
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	38.800, 79.700, 60.800
Unit cell angles	90.00, 102.20, 90.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.191
Rwork	0.191
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT AND MOLECULAR REPLACEMENT AVERAGING BETWEEN TWO CRYSTAL FORMS
RMSD bond length	0.013
RMSD bond angle	27.500 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.600	2.600
Rmerge	0.047
Total number of observations	19983 *
Number of reflections	7850
Completeness [%]	77.9	23.2 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.3		6-8%(W/V) PEG 8000, 300 MM TRIS, PH8.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3-4 (mg/ml)
2	1	drop	PEG8000	3-4 (%(w/v))
3	1	drop	Tris-HCl	25-150 (mM)
4	1	reservoir	PEG8000	6-8 (%(w/v))
5	1	reservoir	Tris-HCl	50-300 (mM)