2SIC
REFINED CRYSTAL STRUCTURE OF THE COMPLEX OF SUBTILISIN BPN' AND STREPTOMYCES SUBTILISIN INHIBITOR AT 1.8 ANGSTROMS RESOLUTION
Replaces: 1SICExperimental procedure
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.230, 185.900, 69.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.177 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.034 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.047 * |
| Total number of observations | 97766 * |
| Number of reflections | 28118 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 * | used seeding, Hirono, S., (1979) J.Mol.Biol., 131, 855. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | BPN | 50mg | |
| 2 | 1 | 1 | SSI | 17.5mg | |
| 3 | 1 | 1 | Tris-HCl | 0.05 (M) |
