2SGE
GLU 18 VARIANT OF TURKEY OVOMUCOID INHIBITOR THIRD DOMAIN COMPLEXED WITH STREPTOMYCES GRISEUS PROTEINASE B AT PH 10.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.670, 54.840, 45.740 |
| Unit cell angles | 90.00, 119.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sgb |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.600 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.115 |
| Number of reflections | 17051 |
| Completeness [%] | 89.2 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.7 | 0.4M SODIUM/POTASSIUM PHOSPHATE BUFFER AT PH 10.7 |
