2SFP
ALANINE RACEMASE WITH BOUND PROPIONATE INHIBITOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 296 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.700, 90.000, 85.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| Rwork | 0.205 |
| R-free | 0.25900 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1sft |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.386 |
| Total number of observations | 178589 * | |
| Number of reflections | 52775 | |
| <I/σ(I)> | 15.1 | 1.7 |
| Completeness [%] | 87.0 | 54.8 |
| Redundancy | 3.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 10 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | sodium acetate | 200 (mM) | |
| 4 | 1 | drop | PLP | 0.01 (M) | |
| 5 | 1 | drop | BME | 0.1 (%) | |
| 6 | 1 | reservoir | PEG4000 | 20-22 (%) | |
| 7 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 8 | 1 | reservoir | PLP | 0.01 (M) | |
| 9 | 1 | reservoir | BME | 0.01 (%) |
