2SAS

STRUCTURE OF A SARCOPLASMIC CALCIUM-BINDING PROTEIN FROM AMPHIOXUS REFINED AT 2.4 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	59.600, 81.300, 82.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.400
R-factor	0.199
Rwork	0.199
RMSD bond length	0.016
RMSD bond angle	0.045 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.091 *
Total number of observations	45101 *
Number of reflections	8157 *
Completeness [%]	98.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		2.5 (mM)
3	1	drop	ammonium sulfate	54 (%sat)
4	1	drop	Tris-HCl	0.05 (M)
5	1	reservoir	ammonium sulfate	54 (%sat)
6	1	reservoir	Tris-HCl	0.05 (M)