2RTP

STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 3.25, SPACE GROUP I222

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 2 2 2
Unit cell lengths	94.970, 105.400, 47.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.500
R-factor	0.206
Rwork	0.206
R-free	0.24300
RMSD bond length	0.018
RMSD bond angle	4.300 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.570
High resolution limit [Å]	1.260 *	1.500
Rmerge	0.059
Total number of observations	121470 *
Number of reflections	46023
Completeness [%]	65.0	27.3
Redundancy	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)