2RTO

STREPTAVIDIN-2-IMINOBIOTIN COMPLEX, PH 2.6, SPACE GROUP I222

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 2 2 2
Unit cell lengths	95.060, 105.840, 48.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.580
R-factor	0.208
Rwork	0.208
R-free	0.25300
RMSD bond length	0.018
RMSD bond angle	24.100 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.650
High resolution limit [Å]	1.270 *	1.580
Rmerge	0.085
Total number of observations	207279 *
Number of reflections	46358
Completeness [%]	71.0	35.8
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)