2RTF

STREPTAVIDIN-BIOTIN COMPLEX, PH 2.00, SPACE GROUP I222

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 2 2 2
Unit cell lengths	95.130, 105.020, 49.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.470
R-factor	0.208
Rwork	0.208
R-free	0.23000
RMSD bond length	0.018
RMSD bond angle	24.300 *
Data reduction software	bioteX ((MSC))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.540
High resolution limit [Å]	1.260 *	1.470
Rmerge	0.087
Total number of observations	202440 *
Number of reflections	48353
Completeness [%]		39.8
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	2.5 *		ROOM TEMPERATURE, PH 2.0. SYNTHETIC MOTHER LIQUOR OF 75% SATURATED AMMONIUM SULFATE, 25% 1.0 M SODIUM FORMATE, 0.99 MM BIOTIN, 170 MM 2-IMINOBIOTIN, ADJUSTED TO PH 2.00.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	biotin	0.99 (mM)
2	1	1	2-iminobiotin	170 (mM)