2RTA

APOSTREPTAVIDIN, PH 2.97, SPACE GROUP I4122

Experimental procedure

Temperature [K]	273
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 41 2 2
Unit cell lengths	58.150, 58.150, 173.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.390
R-factor	0.206
Rwork	0.206
R-free	0.23800
RMSD bond length	0.019
RMSD bond angle	25.300 *
Data reduction software	bioteX ((MSC))
Data scaling software	bioteX
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.320 *
Rmerge	0.066
Total number of observations	123910 *
Number of reflections	30482
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)