2RN2

STRUCTURAL DETAILS OF RIBONUCLEASE H FROM ESCHERICHIA COLI AS REFINED TO AN ATOMIC RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	44.060, 86.850, 35.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.480
R-factor	0.196
RMSD bond length	0.017
RMSD bond angle	0.036 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.480 *
Rmerge	0.058 *
Total number of observations	17254 *
Completeness [%]	73.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	Tris-HCl	200 (mM)