2RMP
RMP-pepstatin A complex
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 287 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-11 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.520, 50.820, 172.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.28000 |
| Structure solution method | DIRECT ELECTRON DENSITY DIFFERENCE MAP |
| Starting model (for MR) | 2asi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.092 * | 0.159 * |
| Total number of observations | 43156 * | |
| Number of reflections | 9360 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 84.9 | 47.9 * |
| Redundancy | 4.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 3.6 | pH 3.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 26 (%) | |
| 2 | 1 | reservoir | citrate | 20 (mM) | |
| 3 | 1 | drop | RMP | 25 (mg/ml) | |
| 4 | 1 | drop | pepstain A | 0.45 (mg/ml) | |
| 5 | 1 | drop | citrate | 2 (mM) |
