2RLN
THERMODYNAMIC AND STRUCTURAL CONSEQUENCES OF CHANGING A SULPHUR ATOM TO A METHYLENE GROUP IN THE M13NLE MUTATION IN RIBONUCLEASE S
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 44.740, 44.740, 97.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-5 (mg/ml) | |
| 2 | 1 | drop | 3 (M) | ||
| 3 | 1 | drop | acetate | 0.1 (M) | |
| 4 | 1 | drop | ammonium sulfate | 35 (%) |
