2RLD

CRYSTAL STRUCTURE OF A PROTEIN WITH UNKNOWN FUNCTION FROM S23 RIBOSOMAL PROTEIN FAMILY (BT_0352) FROM BACTEROIDES THETAIOTAOMICRON VPI-5482 AT 1.70 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798, 0.9537, 0.9796
Spacegroup name	P 1 21 1
Unit cell lengths	57.238, 83.823, 63.923
Unit cell angles	90.00, 97.42, 90.00

Refinement procedure

Resolution	29.643 - 1.700
R-factor	0.165
Rwork	0.163
R-free	0.20100
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.196
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.643	29.643	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.053	0.033	0.441
Total number of observations		1551	7791
Number of reflections	63158
<I/σ(I)>	9.1	17.4	1.7
Completeness [%]	96.2	96.4	88.2
Redundancy	2	2.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	277	NANODROP, 0.2M CaCl2, 20.0% PEG 3350, no Buffer pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K