2RLD
CRYSTAL STRUCTURE OF A PROTEIN WITH UNKNOWN FUNCTION FROM S23 RIBOSOMAL PROTEIN FAMILY (BT_0352) FROM BACTEROIDES THETAIOTAOMICRON VPI-5482 AT 1.70 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798, 0.9537, 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.238, 83.823, 63.923 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 29.643 - 1.700 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.196 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.643 | 29.643 | 1.740 |
| High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
| Rmerge | 0.053 | 0.033 | 0.441 |
| Total number of observations | 1551 | 7791 | |
| Number of reflections | 63158 | ||
| <I/σ(I)> | 9.1 | 17.4 | 1.7 |
| Completeness [%] | 96.2 | 96.4 | 88.2 |
| Redundancy | 2 | 2.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 277 | NANODROP, 0.2M CaCl2, 20.0% PEG 3350, no Buffer pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
