2RL9
Crystal Structure cation-dependent mannose 6-phosphate receptor at pH 6.5 bound to trimannoside
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-24 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 92.628, 92.628, 85.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
Rwork | 0.199 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 10608 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 292 | 0.1M cacodylate, 25% PEG5000MME, 0.2M ammonium sulfate, pH 6.5, vapor diffusion, temperature 292K |