2RKN

X-ray structure of the self-defense and signaling protein DIR1 from Arabidopsis taliana

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.28230, 1.28310, 1.2770
Spacegroup name	P 21 21 21
Unit cell lengths	28.655, 48.217, 54.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.920 - 1.600
R-factor	0.192
Rwork	0.189
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	2.229
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.920
High resolution limit [Å]		1.820
Rmerge	0.073
Number of reflections	10412
<I/σ(I)>	31.7
Completeness [%]	99.6	100
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.08M acetate buffer pH 6.0, 0.02M ZnSO4, 22% PEG 600 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.08M acetate buffer pH 6.0, 0.02M ZnSO4, 22% PEG 600 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.08M acetate buffer pH 6.0, 0.02M ZnSO4, 22% PEG 600 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K