2RKL
Crystal Structure of S.cerevisiae Vta1 C-terminal domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Detector technology | CCD |
| Collection date | 2006-07-19 |
| Wavelength(s) | 0.9793, 0.9794, 0.9566 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.420, 50.707, 74.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.062 | 0.054 | 0.202 |
| Number of reflections | 59057 | ||
| <I/σ(I)> | 16.9 | ||
| Completeness [%] | 94.2 | 82.8 | 91.1 |
| Redundancy | 4 | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | MPD, vapor diffusion, temperature 277K |
