2RKK
Crystal Structure of S.cerevisiae Vta1 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 126.169, 126.169, 70.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.215 |
| R-free | 0.26000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.108 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.062 | 0.033 | 0.418 |
| Number of reflections | 12169 | ||
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.5 | 99.3 | 96.7 |
| Redundancy | 7.4 | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | Sodium Formate, vapor diffusion, temperature 293K, pH 7, VAPOR DIFFUSION |
