2RKH

Crystal structure of a putative AphA-like transcription factor (ZP_00208345.1) from Magnetospirillum magnetotacticum MS-1 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000, 0.9795, 0.9797
Spacegroup name	P 43 21 2
Unit cell lengths	66.620, 66.620, 92.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.364 - 2.000
R-factor	0.214
Rwork	0.212
R-free	0.25600
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.456
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.364	28.364	2.070
High resolution limit [Å]	2.000	4.300	2.000
Rmerge	0.046	0.023	0.665
Number of reflections	14688	2724	2578
<I/σ(I)>	16.26	42.9	2
Completeness [%]	99.7	99.4	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	NANODROP, 15.0% Glycerol, 0.17M NaOAc, 25.5% PEG 4000, 0.1M Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K