2RK8
The Structure of rat cytosolic PEPCK in complex with phosphonoformate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-16 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.251, 119.470, 86.870 |
| Unit cell angles | 90.00, 107.06, 90.00 |
Refinement procedure
| Resolution | 30.060 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.24900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.715 | |
| Number of reflections | 80055 | |
| Completeness [%] | 98.2 | 92.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 12-30% PEG 3350, 0.1M HEPES,10 MM MNCL2, pH 7.4, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
