2RK5
Crystal structure of a domain of the putative hemolysin from Streptococcus mutans UA159
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 34.490, 40.598, 57.630 |
| Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
| Resolution | 57.640 - 1.500 |
| R-factor | 0.18182 |
| Rwork | 0.179 |
| R-free | 0.23684 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.477 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.640 | 1.539 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.074 | 0.245 |
| Number of reflections | 12082 | |
| <I/σ(I)> | 20.4 | 4.3 |
| Completeness [%] | 98.7 | 92.53 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M Hepes pH 7.5, 0.05M MgCl2, 30% PEG MME350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
