2RK0
Crystal structure of glyoxylase/bleomycin resistance protein/dioxygenase domain from Frankia sp. EAN1pec
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 69.445, 69.445, 213.958 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.970 - 2.040 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.25600 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.092 | 0.482 |
Number of reflections | 20212 | |
<I/σ(I)> | 14.2 | 3 |
Completeness [%] | 98.6 | 86.5 |
Redundancy | 32.6 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 1.4M Sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |