2RJV
Crystal structure of the H41Y mutant of villin headpiece, P 21 21 21 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.364, 36.995, 54.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.440 - 1.450 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 1YU5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 11642 | |
<I/σ(I)> | 36.0579 | 6.877 |
Completeness [%] | 98.6 | 95.4 |
Redundancy | 4.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG8000, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |