2RJN
Crystal structure of an uncharacterized protein Q2BKU2 from Neptuniibacter caesariensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.474, 52.973, 55.124 |
| Unit cell angles | 90.00, 124.96, 90.00 |
Refinement procedure
| Resolution | 19.230 - 2.100 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.867 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.059 | 0.046 | 0.188 |
| Number of reflections | 19935 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 79.9 | 98.9 | 42.4 |
| Redundancy | 3.5 | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 4 M Sodium formate, 0.01M Spermine tetrachloride, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
