2RJM
3Ig structure of titin domains I67-I69 E-to-A mutated variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.280, 86.480, 44.410 |
| Unit cell angles | 90.00, 104.94, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.22800 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2rik |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.472 |
| Number of reflections | 20955 | 533 |
| <I/σ(I)> | 13.77 | 3.2 |
| Completeness [%] | 99.2 | 84.3 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | pH 4.6, vapor diffusion, hanging drop, temperature 298K |
