2RJ7
B-specific alpha-1,3-galactosyltransferase G176R S235G mutant (AABB) + UDPGal + Deoxy-acceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-10 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.360, 148.650, 79.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.434 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.880 | 19.880 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.057 | 0.034 | 0.310 |
| Total number of observations | 14543 | 12142 | |
| Number of reflections | 32932 | ||
| <I/σ(I)> | 11.5 | 28.8 | 3.3 |
| Completeness [%] | 95.3 | 91.2 | 98.9 |
| Redundancy | 3.94 | 4.34 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 MPD MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
