2RJ4
B-specific alpha-1,3-galactosyltransferase \ G176R +UDP+ADA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-27 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.480, 149.740, 79.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.470 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.950 | 19.950 | 1.520 |
| High resolution limit [Å] | 1.470 | 3.160 | 1.470 |
| Rmerge | 0.034 | 0.019 | 0.289 |
| Total number of observations | 27586 | 18910 | |
| Number of reflections | 53049 | ||
| <I/σ(I)> | 20 | 60.3 | 3.8 |
| Completeness [%] | 98.6 | 94.8 | 97.8 |
| Redundancy | 4.47 | 5.06 | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
