2RJ1
B-specific alpha-1,3-galactosyltransferase (GTB) G176R mutant + UDP + H-antigen disaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-20 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.450, 149.120, 79.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.550 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.22900 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.353 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.4SSI) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.740 | 19.740 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.330 | 1.550 |
| Rmerge | 0.046 | 0.023 | 0.332 |
| Total number of observations | 22067 | 15707 | |
| Number of reflections | 44381 | ||
| <I/σ(I)> | 15.7 | 46.6 | 3.4 |
| Completeness [%] | 97.1 | 98.7 | 94.2 |
| Redundancy | 4.38 | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.5 | 298 | PEG4000 Glycerol MgCl NH2SO4, pH 7.5, hanging drop, temperature 298K |
