2RIR
Crystal structure of dipicolinate synthase, A chain, from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-09 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.578, 75.108, 200.464 |
| Unit cell angles | 90.00, 119.74, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.790 |
| R-factor | 0.2011 |
| Rwork | 0.199 |
| R-free | 0.24940 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.146 | 0.774 |
| Number of reflections | 81355 | |
| <I/σ(I)> | 5.5 | 1.37 |
| Completeness [%] | 97.0 | 88.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.2 M Lithium sulfate, 0.1 M Tris buffer, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
