2RIN
ABC-transporter choline binding protein in complex with acetylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.200, 212.700, 42.800 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.027 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.055 |
| Number of reflections | 51357 | |
| <I/σ(I)> | 14.89 | 4.42 |
| Completeness [%] | 93.9 | 93.9 |
| Redundancy | 1.87 | 1.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M Na-acetate pH 5.0, 16% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
