2RIF
CBS domain protein PAE2072 from Pyrobaculum aerophilum complexed with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 55.726, 55.726, 336.351 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.910 - 2.350 |
| R-factor | 0.24218 |
| Rwork | 0.239 |
| R-free | 0.29422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.137 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.040 | 2.350 |
| Rmerge | 0.064 | 0.037 | 0.373 |
| Number of reflections | 21965 | ||
| <I/σ(I)> | 13.3 | ||
| Completeness [%] | 93.7 | 84.7 | 99.6 |
| Redundancy | 2.6 | 2.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 7.8 | 298 | PEG3350, Lithium Acetate, Cesium Chloride, pH 7.8, hanging drop vapor diffusion, temperature 298K |
