2RHZ

Crystal structure of the 3-MBT repeats from human L3MBTL1 with D355N point mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-06-24
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	86.015, 91.176, 58.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.223
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rhy
RMSD bond length	0.006
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.082	0.496
Number of reflections	23992
<I/σ(I)>	23.4	4.8
Completeness [%]	100.0	100
Redundancy	6.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	5% PEG10K, 0.1 M Tris-maleate pH 6.0, 0.1 M Na-K-Tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K