2RH7
Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis
Replaces: 2PSLExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Collection date | 2005-09-17 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.744, 85.405, 158.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| Rwork | 0.291 |
| R-free | 0.32500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | hybrid of pdb entries 1MOV 1mou 1XSS and 1GGX |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 123752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.0M ammonium sulfate, 5% v/v isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
