2RH5
Structure of Apo Adenylate Kinase from Aquifex Aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.681, 157.596, 84.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 2.480 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.25500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.268 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.480 | 2.500 |
| Rmerge | 0.076 | 0.885 |
| Number of reflections | 24570 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 96.4 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 28-30% (w/v) PEG 3000, 200 mM sodium acetate, and 100 mM TRIS, pH 8.5, VAPOR DIFFUSION, temperature 298K |
