2RH3
Crystal structure of plasmid pTiC58 VirC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979602 |
| Spacegroup name | P 31 |
| Unit cell lengths | 57.374, 57.374, 33.935 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.08) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.062 | 0.620 |
| Number of reflections | 13376 | |
| <I/σ(I)> | 18.6 | 18.6 |
| Completeness [%] | 96.9 | 96.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
