2RH0

Crystal structure of NudC domain-containing protein 2 (13542905) from Mus musculus at 1.95 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-19
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97942
Spacegroup name	P 1
Unit cell lengths	44.568, 64.504, 64.423
Unit cell angles	74.51, 81.06, 81.22

Refinement procedure

Resolution	29.148 - 1.950
R-factor	0.192
Rwork	0.190
R-free	0.23500
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.610
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.148	29.148	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.096	0.060	0.607
Total number of observations		1477	10099
Number of reflections	47221
<I/σ(I)>	3.1	8.2	0.8
Completeness [%]	95.5	94.7	94.2
Redundancy	2.9	2.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	NANODROP, 0.2M NaNO3, 20.0% PEG 3350, No Buffer pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K