2RGR
Topoisomerase IIA bound to G-segment DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3776 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 85.435, 126.801, 223.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.873 - 3.000 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 1BGW 1i7d |
| RMSD bond length | 1.402 |
| RMSD bond angle | 0.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.086 | 0.028 | 0.438 |
| Number of reflections | 49135 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.6 | 98.7 | 98.4 |
| Redundancy | 2.2 | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 12-20% PEG 1000, 100-250 mM MgCl2, 100 mM sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 1000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | sodium cacodylate | ||
| 4 | 1 | 2 | PEG 1000 | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | sodium cacodylate |
