2RGH

Structure of Alpha-Glycerophosphate Oxidase from Streptococcus sp.: A Template for the Mitochondrial Alpha-Glycerophosphate Dehydrogenase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9783, 0.979, 0.96
Spacegroup name	P 21 21 2
Unit cell lengths	86.280, 95.550, 77.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.760 - 2.300
R-factor	0.211
Rwork	0.210
R-free	0.25300
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.790	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	37395
<I/σ(I)>	12.9	4
Completeness [%]	97.7	82.4
Redundancy	6.49	4.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	sodium sulfate, PEG 3350, beta-mercaptoethanol, glycerol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K