2RGH
Structure of Alpha-Glycerophosphate Oxidase from Streptococcus sp.: A Template for the Mitochondrial Alpha-Glycerophosphate Dehydrogenase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9783, 0.979, 0.96 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.280, 95.550, 77.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.760 - 2.300 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.790 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 37395 | |
| <I/σ(I)> | 12.9 | 4 |
| Completeness [%] | 97.7 | 82.4 |
| Redundancy | 6.49 | 4.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | sodium sulfate, PEG 3350, beta-mercaptoethanol, glycerol, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
