2RG7
Apo- Crystal Structure of a Periplasmic Heme Binding Protein from Shigella dysenteriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.817, 73.280, 72.380 |
| Unit cell angles | 71.44, 77.80, 89.90 |
Refinement procedure
| Resolution | 35.900 - 2.050 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r7a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.900 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.057 | 0.666 |
| Number of reflections | 63384 | |
| <I/σ(I)> | 27.19 | 2.01 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.1m SODIUM CITRATE, 0.05m AMMONIUM BROMIDE, 15% POLYETHYLENE GLYCOL 4000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
