2RG2
Crystal structure of variant R18L of conjugated bile acid hydrolase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-23 |
| Detector | Mar 345 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 64.244, 64.244, 170.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21300 |
| Starting model (for MR) | 2bjfA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.207 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.052 | 0.243 |
| Number of reflections | 35363 | |
| <I/σ(I)> | 14.4 | 8.3 |
| Completeness [%] | 98.9 | 89.2 |
| Redundancy | 11.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100 mM Tris pH 8.5, 200 mM lithium sulfate, 25% PEG 3350, vapor diffusion, hanging drop, temperature 291K |
