2RFR

Crystal structure of an ntf2-like protein with a cystatin-like fold (saro_3722) from novosphingobium aromaticivorans dsm at 1.16 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9797, 1.0000
Spacegroup name	P 3 2 1
Unit cell lengths	63.135, 63.135, 75.118
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.338 - 1.160
R-factor	0.142
Rwork	0.141
R-free	0.17200
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.747
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.338	27.338	1.190
High resolution limit [Å]	1.160	5.190	1.160
Rmerge	0.085	0.038	0.618
Total number of observations		3726	12790
Number of reflections	60052
<I/σ(I)>	5.6	15.9	1.2
Completeness [%]	99.5	99.2	94.4
Redundancy	4.7	4.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	NANODROP, 20.0% PEG 6000, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K