2RFQ
Crystal structure of 3-HSA hydroxylase from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.283, 76.137, 80.881 |
| Unit cell angles | 83.78, 75.95, 89.99 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.14741 |
| Rwork | 0.146 |
| R-free | 0.17127 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.088 | 0.334 |
| Number of reflections | 196906 | |
| <I/σ(I)> | 24.75 | 3.31 |
| Completeness [%] | 97.3 | 95 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2M NaCl, 0.1M Hepes pH 7.5, 25% PEG 3350, 0.3M PPS, 2mM L-Cysteine, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
