2RFF
Crystal structure of a putative nucleotidyltransferase (NP_343093.1) from Sulfolobus solfataricus at 1.40 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.007, 29.884, 35.202 |
| Unit cell angles | 77.24, 73.72, 78.30 |
Refinement procedure
| Resolution | 33.278 - 1.400 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.18700 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.807 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.278 | 33.278 | 1.450 |
| High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
| Rmerge | 0.064 | 0.046 | 0.261 |
| Number of reflections | 16758 | ||
| <I/σ(I)> | 13 | ||
| Completeness [%] | 90.5 | 99 | 54 |
| Redundancy | 3.7 | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | NANODROP, 20.0% PEG MME2000, 0.1M Tris-HCl pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
