2RFC
Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.520, 141.010, 101.030 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 41.850 - 3.100 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.33400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.850 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.122 | 0.122 |
| Number of reflections | 23827 | |
| <I/σ(I)> | 8.72 | 1.93 |
| Completeness [%] | 95.4 | 98.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.05M sodium chloride, 0.1M HEPES, 30% polyethyleneglycol 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
