2REY
Crystal structure of the PDZ domain of human dishevelled 2 (homologous to Drosophila dsh)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.644, 69.644, 57.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.820 - 1.550 |
| R-factor | 0.21217 |
| Rwork | 0.211 |
| R-free | 0.23982 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.549 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.820 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.046 | 0.468 |
| Number of reflections | 12446 | |
| <I/σ(I)> | 15.3 | 5.6 |
| Completeness [%] | 99.6 | 96.1 |
| Redundancy | 14.9 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M Ammonium acetate, 0.1M Bis-tris pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
