2REJ

ABC-transporter choline binding protein in unliganded semi-closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Detector technology	CCD
Collection date	2006-12-06
Detector	MAR CCD 165 mm
Wavelength(s)	0.90
Spacegroup name	P 1 21 1
Unit cell lengths	34.100, 232.500, 47.300
Unit cell angles	90.00, 90.20, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.29889
Rwork	0.296
R-free	0.35867
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Rmerge	0.222
Number of reflections	22282
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M Na acetate pH 5.0, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K