2REG
ABC-transporter choline binding protein in complex with choline
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.796, 185.414, 42.529 |
| Unit cell angles | 90.00, 91.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.18172 |
| Rwork | 0.180 |
| R-free | 0.22242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 37385 |
| Redundancy | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 0.1M Na acetate pH 4.6, 18% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
