2RE7

Crystal structure of a pyridoxamine 5'-phosphate oxidase related protein (psyc_0186) from psychrobacter arcticus 273-4 at 2.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.95372, 0.97942
Spacegroup name	I 41 2 2
Unit cell lengths	106.063, 106.063, 87.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.433 - 2.500
R-factor	0.192
Rwork	0.191
R-free	0.22600
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.645
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.433	28.433	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.090	0.010	0.010
Total number of observations		678	4683
Number of reflections	8894
<I/σ(I)>	4.2	7.5	0.7
Completeness [%]	99.9	95.3	100
Redundancy	7.2	5.7	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	NANODROP, 3.2M (NH4)2SO4, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K